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Invitrogen
Folic Acid Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
0.94 ng/mL
Assay range
1.56-100 ng/mL
Sample type/volume
Plasma
50 µL
Tissue Homogenate
50 µL
Serum
50 µL
Cell Lysate
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Folic Acid
Species (tested)
All
Species (reported)
All
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Folic Acid/Vitamin B9 (FA/VB9) ELISA quantitates FA/VB9 in serum, plasma and other biological fluids.
Principle of the method
The Competitive multispecies ELISA research use only kit is designed to quantitatively measure Folic Acid/Vitamin B9 (FA/VB9) independent of species. A FA/VB9 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to FA/VB9 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of FA/VB9 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Folic acid, a B-vitamin essential for numerous biochemical processes, plays a key role in DNA synthesis and repair, as well as methylation, which is crucial for genome regulation and normal mammalian development. It functions primarily as a methyl-group donor and is involved in critical biological processes such as cell division and gene expression regulation. This vitamin is integral to folate metabolism, which has significant implications for DNA methylation and thereby impacts epigenetic modifications. Variations in dietary intake and functional polymorphisms in the genes encoding folate metabolism enzymes can influence its efficacy, making it a potential factor in cancer susceptibility and other diseases. Folic acid deficiency is common and can arise from poor diet, malabsorption, alcohol use, obesity, and kidney failure. Due to its protective effects against neural tube defects and ischemic events, folic acid fortification in foods has been adopted in many countries. The molecular interaction of folic acid with DNA and RNA occurs at different sites, highlighting its multifaceted role in cellular processes. Overall, folic acid's impact on DNA methylation underscores its importance in health and disease prevention.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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