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Invitrogen
Human Aminopeptidase N/ANPEP ELISA Kit
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Product Specifications
Analytical sensitivity
0.041 ng/mL
Assay range
0.041-10 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
CD13
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human Aminopeptidase N/ANPEP quantitates human Aminopeptidase N/ANPEP in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Aminopeptidase N/ANPEP.
Target information
CD13, also known as ANPEP, is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on a wide variety of cell types and tissues. It is prominently found on monocytes and granulocytes in the blood, but not on lymphocytes. Additionally, CD13 is expressed on endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, placenta, and myeloid leukemia cells. As a metalloprotease, CD13 preferentially catalyzes the removal of neutral amino acids from small peptides, thereby activating or inactivating bioactive peptides. It plays a crucial role in various biological processes, including the degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and the adhesion and migration of cells. CD13 is also involved in extracellular matrix degradation, signal transduction, inflammatory responses, regulation of intercellular contact, cell motility, and vascularization. In the context of disease, CD13 is associated with the protection of leukemic cells against apoptosis and is linked to a poor prognosis in carcinomas. Furthermore, CD13 functions as an aminopeptidase enzyme and serves as an alpha receptor for coronavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ANPEP, APN, CD13, GP150, LAP1, P150, PEPN
Gene ID : (Human) 290
Gene symbol : ANPEP
Protein Aliases : alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, AP-M, AP-N, CD13, gp150, h AP-N, hAPN, membrane alanyl aminopeptidase, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13
UniProt ID (Human) P15144
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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