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Invitrogen
Human CD5L Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.09 ng/mL
Assay range
0.16-10 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
CD5L
Species (tested)
Human
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human CD5L (CD5 Antigen-like) ELISA quantitates CD5L in serum and plasma.
Principle of the method
The Human CD5 Antigen-like (CD5L) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of CD5 Antigen-like (CD5L) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to CD5 Antigen-like (CD5L). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of CD5 Antigen-like (CD5L) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
CD5L, also known as CD5 antigen-like, is a protein that is expressed on the surface of various immune cells, including T cells, B cells, and natural killer cells. It is involved in the regulation of immune responses and is being studied as a potential therapeutic target in various diseases, including autoimmune disorders, cancer, and infectious diseases. CD5L is known to modulate T cell activation and cytokine production, and has been shown to have anti-inflammatory effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AIM, API6, CT-2, hAIM, PRO229, SP-ALPHA, Spalpha
Gene ID : (Human) 922
Gene symbol : CD5L
Protein Aliases : AIM, APOPTOSIS INHIBITOR OF MACROPHAGES, CD5 antigen like (scavenger receptor cysteine rich family), Highly similar to ANTIGEN WC1.1 [Bos taurus], SCAVENGER RECEPTOR CYSTEINE-RICH FAMILY, apoptosis inhibitor 6, apoptosis inhibitor expressed by macrophages, formerly named CT-2, apoptosis inhibitor of macrophage, CD5 antigen-like (scavenger receptor cysteine rich family), igM-associated peptide, unnamed protein product
UniProt ID (Human) O43866
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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