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Invitrogen
Human Fc epsilon RI ELISA Kit
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Product Specifications
Analytical sensitivity
<0.068 ng/mL
Assay range
0.078-5 ng/mL
Sample type/volume
Serum
20 µL
Plasma
20 µL
Supernatant
20 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 30 min
Homogenous (no wash)
No
Interassay CV
6.8%
Intraassay CV
6.1%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Detection antibody (chimeric IgE)
IgE - HRP conjugate
Assay Buffer concentrate
Wash Buffer concentrate
Substrate solution
Stop Solution
Adhesive Plate Covers
Standard
Detection antibody (chimeric IgE)
IgE - HRP conjugate
Assay Buffer concentrate
Wash Buffer concentrate
Substrate solution
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
FceR1 alpha
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Fc epsilon RI ELISA quantitates Fc epsilon RI in human serum, plasma, and cell culture medium.
Principle of the method
The Human Fc epsilon RI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Fc epsilon RI alpha, also known as FceR1 alpha, is a subunit of the high-affinity receptor for IgE, primarily expressed on mast cells and basophils. Its expression is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits, which are essential for signal transduction. The beta and gamma subunits contain immunoreceptor tyrosine-based activation motifs (ITAMs). The Fc epsilon RI complex plays a crucial role in triggering IgE-mediated allergic reactions. When allergen-bound IgE molecules bring together two or more high-affinity IgE receptors, it leads to the release of mediators such as histamine, which are responsible for allergy symptoms. This process couples allergens and mast cells to initiate inflammatory responses characteristic of allergic disorders like hay fever and asthma. The release of histamine and proteases also leads to the synthesis of prostaglandins and leukotrienes, which are potent effectors of the hypersensitivity response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : FCE1A, FCER1A, FcERI
Gene ID : (Human) 2205
Gene symbol : FCER1A
Protein Aliases : fc epsilon r1, RP11-550P17.3, Fc epsilon receptor Ia, Fc epsilon RI alpha-chain, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, Fc IgE receptor, Fc-epsilon RI-alpha, FcERI, high affinity immunoglobulin epsilon receptor alpha-subunit, High affinity immunoglobulin epsilon receptor subunit alpha, IgE Fc receptor subunit alpha, immunoglobulin E receptor, high-affinity, of mast cells
UniProt ID (Human) P12319
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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