Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

18.75 pg/mL

Assay range

31.25-2000 pg/mL

Sample type/volume

Serum
100 µL
Plasma
100 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Micro ELISA Plate (Dismountable)
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

GPX1

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human GPX1 ELISA Kit quantifies GPX1 in serum and plasma.

The Human GPX1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Target information

Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : GPX1, GPXD, GSHPX1

Gene ID : (Human) 2876

Gene symbol : GPX1

Protein Aliases : Cellular glutathione peroxidase, Glutathione peroxidase 1, GPx-1, HGNC:4553, MGC14399, MGC88245, OTTHUMP00000210766, Phospholipid-hydroperoxide glutathione peroxidase GPX1

UniProt ID (Human) P07203

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