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Invitrogen
Human Ghrelin ELISA Kit
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Product Specifications
Analytical sensitivity
11.8 pg/mL
Assay range
15.6-1,000.0 pg/mL
Sample type/volume
Plasma
25 µL
Serum
25 µL
Supernatant
25 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
8.5%
Intraassay CV
6%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Ghrelin
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Ghrelin ELISA quantitates Hu Ghrelin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Ghrelin.
Principle of the method
The Human Ghrelin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an the ligand for the growth hormone secretagogue receptor type 1 and is involved in regulating growth hormone release. Ghrelin has an appeptite-stimulating effect and induces adiposity and stimulates gastric acid secretion. Obestatin may be the ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and can have an appetite-reducing effect resulting in decreased food intake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : GHRL, MTLRP, UNQ524/PRO1066
Gene ID : (Human) 51738
Gene symbol : GHRL
Protein Aliases : AMCBX1, FIP3, FIP-3, Fip3p, ghrelin 27, ghrelin 28, NEMO, OTTHUMP00000207794, OTTHUMP00000207795, OTTHUMP00000207796, OTTHUMP00000207798, ghrelin/obestatin, IKK-gamma, IP, IP1, IP2, IPD2, Appetite-regulating hormone, ghrelin, growth hormone secretagogue receptor ligand, ghrelin/obestatin preprohormone, Growth hormone secretagogue, Growth hormone-releasing peptide, In2c-preproghrelin, Motilin-related peptide, prepro-appetite regulatory hormone, preproghrelin, Protein M46
UniProt ID (Human) Q9UBU3
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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