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Invitrogen
Human IFN omega Matched Antibody Pair
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Product Specifications
Analytical sensitivity
4.7 pg/mL
Assay range
4.7-300 pg/mL
Sample type/volume
Plasma
25 µL
Serum
25 µL
Supernatant
25 µL
Hands-on time
24 hr
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
10 x 96 Tests
Contents
Capture (coating) Antibody
Detection Antibody
Standard
Sample Diluent
Detection Antibody
Standard
Sample Diluent
Shipping conditions
Wet ice
Storage
-20°C
Protein name
IFN omega
Target site-specificity
Assay kit format
Matched Antibody Pair
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human IFN-β Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, sample diluent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IFN-β. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
IFN-omega is a type I interferon that can be induced by virus-infected leukocytes. Members of the type I interferon family, which includes IFN-alpha, IFN-beta, and IFN-omega, signal through the IFNAR-1/IFNAR-2 receptor complex, and exert antiviral and antiproliferative activities. IFN-omega exhibits about 75% sequence homology with IFN-alpha, and contains two conserved disulfide bonds that are necessary for full biological activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Interferon alpha-II-1
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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