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Invitrogen
Human IL-18 Matched Antibody Pair
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Product Specifications
Analytical sensitivity
78 pg/mL
Assay range
78-5,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
2 hr 10 min
Time-to-result
24 hr
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
10 x 96 Tests
Contents
Capture (coating) Antibody
Detection Antibody
SAV-HRP
Standard
Sample Diluent
Detection Antibody
SAV-HRP
Standard
Sample Diluent
Shipping conditions
Wet ice
Storage
-20°C
Protein name
IL-18
Species (tested)
Human
Assay kit format
Matched Antibody Pair
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human IL-18 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, sample diluent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-18. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : IGIF, IL-18, IL-1g, IL18, IL1F4
Gene ID : (Human) 3606
Gene symbol : IL18
Protein Aliases : Iboctadekin, IFN-gamma-inducing factor, il 18, ILN, Interleukin, Interleukin18, MGC12320, IL-1 gamma, IL-18, Interferon gamma-inducing factor, interleukin 18 (interferon-gamma-inducing factor), Interleukin-1 gamma, Interleukin-18
UniProt ID (Human) Q14116
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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