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Invitrogen
Human IL-27 ELISA Kit
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Product Specifications
Analytical sensitivity
81 pg/mL
Assay range
81-20,000 pg/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
IL-27
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human IL-27 quantitates human IL-27 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human IL-27.
Target information
IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : EBI3, IL-27, IL-27A, IL-27B, IL27, IL27A, IL27B, IL27p28, IL30, p28
Gene ID : (Human) 10148, (Human) 246778
Gene symbol : EBI3, IL27
Protein Aliases : cytokine receptor, EBV-induced gene 3 protein, Epstein-Barr virus induced gene 3, Epstein-Barr virus-induced gene 3 protein, IL-27 p28 subunit, IL-27 subunit alpha, IL-27 subunit beta, IL-27-A, IL27 p28, ILN, Interleukin, MGC71873, M-IL-27 (p28), IL27 subunit, IL27-A, IL35 subunit, interleukin 30, Interleukin-27 subunit alpha, Interleukin-27 subunit beta, Interleukin-30, p28
UniProt ID (Human) Q14213, (Human) Q8NEV9
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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