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Invitrogen
Human IL-7 R alpha/CD127 ELISA Kit
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Product Specifications
Analytical sensitivity
0.14 ng/mL
Assay range
0.14-100 ng/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
CD127
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human IL-7 R alpha/CD127 quantitates human IL-7 R alpha/CD127 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human IL-7 R alpha/CD127.
Target information
CD127, also known as the Interleukin-7 receptor alpha chain (IL-7Ralpha), is a type I glycoprotein with a molecular weight of 75-80 kDa. It forms a multi-functional receptor complex with CD132, the common gamma chain, to create the IL-7 receptor (IL-7R), which is crucial for lymphopoiesis. The active IL-7 receptor is an alpha/gamma chain heterodimer, where the gamma chain also associates with the interleukin-2 receptor and primarily activates signal transduction, while the alpha chain determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 is expressed by immature B cells through the early pre-B stage, thymocytes during various stages of development, and most mature T cells, with transient down-regulation upon activation. It promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. Binding of IL-7 to CD127 results in signal transduction through several tyrosine kinase pathways, including the Jak/STAT pathway, and is indispensable for lymphocyte development and the control of homeostatic proliferation of T cells in the periphery. Additionally, IL-7R signaling is involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells. Interestingly, CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While CD4 and CD25 co-expression is commonly used to identify T regs, this method may include cells without suppressive activity. CD4+CD25+ cells that have down-regulated CD127 expression are significantly enriched for the regulatory T cell population, as defined by the expression of the T reg-specific transcription factor Foxp3 and their suppressive activity in vitro. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CD127, CDW127, IL-7R-alpha, IL-7Ralpha, IL7R, IL7RA, IL7Ralpha, ILRA, IMD104, lnc-IL7R, sIL-7R
Gene ID : (Human) 3575
Gene symbol : IL7R
Protein Aliases : CD127, CD127 antigen, CDw127, IL 7 receptor, IL 7R a, IL 7R subunit alpha, IL 7R α, IL 7Ra, IL 7Rα, soluble IL 7R, IL7R α, IL7Rα, Interleukin7 receptor subunit alpha, MGC107557, sCD127, soluble IL 7 receptor, IL7 receptor, IL7 receptor subunit alpha, IL-7R, IL7R a, IL7R alpha, IL7R subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL7R, IL-7 receptor alpha chain, interleukin 7 receptor alpha chain, interleukin-7 receptor precursor, Interleukin-7 receptor subunit alpha, soluble interleukin-7 receptor, unnamed protein product
UniProt ID (Human) P16871
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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