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Invitrogen
Human/Mouse BCL-W ELISA Kit
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Product Specifications
Analytical sensitivity
0.41 ng/mL
Assay range
0.41-100 ng/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
BCL-W
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Mouse BCL-W quantitates mouse BCL-W in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse BCL-W.
Target information
Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRalpha gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues. An alternative splicing event in exon 4 results in two transcripts. The first, Bcl-w, encodes a protein of 193 amino acids, and the second, Bcl-w/rox, encodes a protein 333 amino acids in length. The "rox" portion of Bcl-w/rox shows a striking 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : AW048834, BCL-W, BCL2-L-2, BCL2L2, BCLW, c98, Gtrgal2, Gtrosa41, KIAA0271, PPP1R51
Gene ID : (Human) 599, (Mouse) 12050
Gene symbol : BCL2L2, Bcl2l2
Protein Aliases : Apoptosis regulator Bcl-W, Bcl-2-like protein 2, Bcl2-L-2, BCL2-like 2 protein, Bcl2-like protein 2, c98, protein phosphatase 1, regulatory subunit 51
UniProt ID (Human) Q92843, (Mouse) P70345
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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