Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.4 ng/mL

Assay range

0.4-100 ng/mL

Sample type/volume

Supernatant
50 µL
Serum
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

Thymidine Phosphorylase

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human PD-ECGF quantitates human PD-ECGF in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PD-ECGF.

Target information

A platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : ECGF, ECGF1, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PDECGF, TP, TYMP

Gene ID : (Human) 1890

Gene symbol : TYMP

Protein Aliases : Gliostatin, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine phosphorylase, TP

UniProt ID (Human) P19971

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