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Invitrogen
Human RARRES2/TIG2 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.19 ng/mL
Assay range
0.31-20 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Chemerin
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human CHEM (Chemerin) ELISA quantitates CHEM in serum and plasma.
Principle of the method
The Human Chemerin (CHEM) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Chemerin (CHEM) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Chemerin (CHEM). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Chemerin (CHEM) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Retinoids act through ligand-dependent transcription factors, retinoid X receptor (RXRs) and retinoic acid receptors (RARs). Tazarotene-induced gene (TIG) proteins, also designated retinoic acid receptor responder proteins or RAR-responsive proteins, can be membrane bound or secreted. TIGs act as tumor suppressor genes in human cancers and are highly expressed in skin, hair follicles and endothelial cells as well as in pancreas, spleen and intestine. TIGs are activated by tazarotene and have been implicated as growth regulators that mediate the growth suppressive effects of retinoids. TIG2 is a secreted protein that is mainly expressed in epidermis, hair follicles and endothelial cells. TIG2 is inhibited in psoriatic lesions and is activated by tazarotene in skin rafts and in epidermis of psoriatic lesions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : HP10433, RARRES2, TIG2
Gene ID : (Human) 5919
Gene symbol : RARRES2
Protein Aliases : Chemerin, RAR-responsive protein TIG2, RARRES2, retinoic acid receptor responder (tazarotene induced) 2, Retinoic acid receptor responder protein 2, Tazarotene-induced gene 2 protein, TIG-2, TIG2HP10433, unnamed protein product
UniProt ID (Human) Q99969
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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