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Invitrogen
Human TEK ELISA Kit
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Product Specifications
Analytical sensitivity
20 pg/mL
Assay range
20-15,000 pg/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
TIE2 (TEK)
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human TEK ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of human Tie-2 in cell culture supernatants, plasma, and serum samples.
Target information
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD202B, TEK, TIE-2, TIE2, VMCM, VMCM1
Gene ID : (Human) 7010
Gene symbol : TEK
Protein Aliases : Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10, EC 2.7.10.1, TEKT1, Tektin-1, Endothelial tyrosine kinase, hTIE2, p140 TEK, TEK tyrosine kinase, endothelial, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2
UniProt ID (Human) E7EWI2
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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