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Invitrogen
Human Aggrecan (PG) ELISA Kit
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Product Specifications
Analytical sensitivity
0.9 ng/mL
Assay range
10-250 ng/mL
Sample type/volume
Serum
20 µL
Supernatant
10 µL
Synovial Fluid
25 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 15 min
Homogenous (no wash)
No
Interassay CV
5.6%
Intraassay CV
2.1%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample diluent
Incubation Buffer
HRP conjugated Detection Antibody
Conjugate Diluent
Wash Buffer
Chromogen
Stop Solution
Controls
Standard
Sample diluent
Incubation Buffer
HRP conjugated Detection Antibody
Conjugate Diluent
Wash Buffer
Chromogen
Stop Solution
Controls
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Aggrecan
Protein subtype
Proteoglycans
Protein family
Other Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Aggrecan (PG) ELISA quantitates Hu Aggrecan (PG) in humansynovial fluid, serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Aggrecan (PG).
Principle of the method
The Human Aggrecan (PG) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second enzyme-conjugated (detector) antibody, binding to the target on a different epitope from the capture antibody. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : ACAN, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK
Gene ID : (Human) 176
Gene symbol : ACAN
Protein Aliases : Aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, large aggregating proteoglycan
UniProt ID (Human) Q9UCP4
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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