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Invitrogen
Mouse IL-10 ELISA Kit
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Product Specifications
Analytical sensitivity
5.0 pg/mL
Assay range
39-2,500 pg/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
5.1%
Intraassay CV
4.4%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-10
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
Biotin
About This Kit
The mouse IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-10.
Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. It can block cytokine synthesis and several accessory cell functions of macrophages, T-cells, and NK cells. In addition, IL-10 participates in the regulation of proliferation and differentiation of B-cells, mast cells, and thymocytes. Murine IL-10 exhibits strong DNA and amino acid sequence homology to human IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1, which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.
Target information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CSIF, Il-10, Il10
Gene ID : (Mouse) 16153
Gene symbol : Il10
Protein Aliases : CSIF, Cytokine synthesis inhibitory factor, H-IL-10, il 10, ILN, Interleukin, Interleukin10, MGC126450, MGC126451, RP11-262N9.1, IL-10, Interleukin-10
UniProt ID (Mouse) P18893
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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