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Product Specifications
Analytical sensitivity
2.97 pg/mL
Assay range
7.8-1000 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Fecal Extract
50 µL
Urine
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<6%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
Testosterone
Protein family
Chromone
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The multispecies Testosterone ELISA with improved sensitivity quantitates testosterone in serum, plasma, dried fecal samples, or urine.
Principle of the method:
The Competitive ELISA research-use-only kit is designed to quantitatively measure testosterone independent of species. A testosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with donkey anti-sheep IgG antibodies. A monoclonal sheep antibodies and testosterone-peroxidase conjugate is added to the wells. The testosterone conjugate and any testosterone in the sample will compete to bind to the sheep testosterone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound testosteron-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of testosterone present in the sample.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone, Testosterone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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