Technical Resources

12551 - Williams' Medium E

Catalog Number(s)
12551032

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75.0	50.0	0.6666667
L-Alanine	89.0	90.0	1.011236
L-Arginine	174.0	50.0	0.28735632
L-Asparagine-H2O	150.0	20.0	0.13333334
L-Aspartic acid	133.0	30.0	0.22556391
L-Cysteine	121.0	40.0	0.3305785
L-Cystine 2HCl	313.0	26.07	0.08329073
L-Glutamic Acid	147.0	50.0	0.34013605
L-Histidine	155.0	15.0	0.09677419
L-Isoleucine	131.0	50.0	0.3816794
L-Leucine	131.0	75.0	0.57251906
L-Lysine hydrochloride	183.0	87.46	0.47792348
L-Methionine	149.0	15.0	0.10067114
L-Phenylalanine	165.0	25.0	0.15151516
L-Proline	115.0	30.0	0.26086956
L-Serine	105.0	10.0	0.0952381
L-Threonine	119.0	40.0	0.33613446
L-Tryptophan	204.0	10.0	0.04901961
L-Tyrosine disodium salt dihydrate	261.0	50.65	0.19406131
L-Valine	117.0	50.0	0.42735043
Vitamins
Ascorbic Acid	176.0	2.0	0.011363637
Biotin	244.0	0.5	0.0020491802
Choline chloride	140.0	1.5	0.010714286
D-Calcium pantothenate	477.0	1.0	0.002096436
Ergocalciferol	397.0	0.1	2.5188917E-4
Folic Acid	441.0	1.0	0.0022675737
Menadione sodium bisulfate	276.0	0.01	3.6231882E-5
Niacinamide	122.0	1.0	0.008196721
Pyridoxal hydrochloride	204.0	1.0	0.004901961
Riboflavin	376.0	0.1	2.6595744E-4
Thiamine hydrochloride	337.0	1.0	0.002967359
Vitamin A (acetate)	328.0	0.1	3.0487805E-4
Vitamin B12	1355.0	0.2	1.4760147E-4
alpha Tocopherol phos. Na salt	554.7	0.01	1.8027762E-5
i-Inositol	180.0	2.0	0.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111.0	200.0	1.8018018
Cupric sulfate (CuSO4-5H2O)	250.0	1.0E-4	3.9999998E-7
Ferric nitrate (Fe(NO3)-9H2O)	404.0	1.0E-4	2.4752475E-7
Magnesium Sulfate (MgSO4) (anhyd.)	120.0	97.67	0.8139166
Manganese chloride (MnCl2-4H2O)	198.0	1.0E-4	5.050505E-7
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Sodium Bicarbonate (NaHCO3)	84.0	2200.0	26.190475
Sodium Chloride (NaCl)	58.0	6800.0	117.24138
Sodium Phosphate monobasic (NaH2PO4) anhydrous	138.0	140.0	1.0144928
Zinc sulfate (ZnSO4-7H2O)	288.0	2.0E-4	6.9444445E-7
Other Components
D-Glucose (Dextrose)	180.0	2000.0	11.111111
Glutathione (reduced)	307.0	0.05	1.6286645E-4
Methyl linoleate	295.0	0.03	1.0169491E-4
Phenol Red	376.4	10.0	0.026567481
Sodium Pyruvate	110.0	25.0	0.22727273

Reference:

1. Williams, G.M. and Gunn, J.M. (1974) Long term cell culture of adult rat liver epithelial cells. Exp. Cell Res., 89:139.

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