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Advanced affinity chromatography for monoclonal antibody therapeutics with POROS and POROS CaptureSelect Columns
Thermo Scientific POROS and POROS CaptureSelect Affinity HPLC columns are specifically designed for the high-throughput separation and analysis of monoclonal antibodies (mAbs). These columns utilize advanced affinity chromatography techniques to offer exceptional binding specificity and capacity for precise and reliable results. An ideal choice for both research and quality control applications, POROS affinity columns offer rapid analysis with high throughput, making them a valuable tool in the development and production of monoclonal antibody therapeutics. Their robust performance and reproducibility can streamline workflows and help enhance the accuracy of analytical processes in biopharmaceutical laboratories.
- High specificity and sensitivity: Excellent separation and detection of complex biomolecules through domain-specific binding
- Fast and efficient: Rapid analysis with high throughput, designed to reduce overall processing time and increase laboratory productivity
- Robust and durable: Engineered for long-lasting performance, these columns are designed to withstand rigorous analytical conditions for consistent results over time
- Versatile applications: Suitable for a wide range of applications, including protein titer quantitation, biomolecule characterization, and quality control
- Enhanced reproducibility: Benefit from consistent and reproducible results to facilitate reliable analytical workflows
Chromatography chemistries
To support the evolving needs of biotherapeutic analysis, Thermo Fisher Scientific offers chromatography chemistries designed for precision, consistency, and efficiency. Explore our POROS resins for high-resolution separations and CaptureSelect ligands for targeted specificity in analytical HPLC workflows.
Explore how POROS resins deliver speed, resolution, and reliability for your most demanding HPLC analyses.
Discover how CaptureSelect ligands bring unmatched specificity and consistency to your analytical workflows.
POROS Protein A & Protein G analytical columns
POROS Protein A and Protein G analytical columns are designed to determine and monitor antibody titer and yield during monoclonal antibody (mAb) development and manufacturing, from samples such as cell culture supernatant, purification process pools, and final formulation.
Protein A columns leverage the high-affinity binding properties of Protein A to selectively capture and analyze mAbs from complex mixtures. Protein A binds to the Fc region of IgG antibodies. It is also effective at binding to the variable region of human IgG molecules (IgG1, IgG2, and IgG4) and fragments with a VH3 domain.
Protein G columns offer broader binding capabilities, including subclasses of IgG such as mouse or rabbit and may be chosen when higher binding affinity is needed. These columns enable precise quantification and characterization of mAbs, facilitating critical quality control applications. Their robust performance aims to deliver reliable, reproducible results, making them an indispensable tool in the development and production of therapeutic antibodies.
POROS CaptureSelect columns for non-traditional mAb analysis
POROS CaptureSelect affinity columns for mAb analysis offer an innovative solution for the characterization of complex, non-traditional antibodies. Utilizing specialized CaptureSelect ligands on a robust POROS bead matrix, this technology offers high specificity, superior binding capacity, and rapid mass transfer, making it an ideal choice for high-throughput and high-resolution analytical applications in bioprocessing workflows.
CaptureSelect ligands offer a unique solution for affinity chromatography. Based on a Camelid-derived single domain [VHH] antibody fragment, these small affinity ligands offer a platform solution for many affinity HPLC challenges. Tailored to bind to specific regions of an antibody, our solution can offer milder elution conditions or fragment analysis without relying on Protein A binding domains.
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For research and development use only in support of FDA-regulated end uses. Not for diagnostic use or direct administration to humans or animals.