LinkSēq™ Blood Genotyping

Real-Time PCR for RBC, ABO and HPA Genotyping

Fast, Easy, Comprehensive Molecular Antigen Typing using real-time PCR

LinkSēq real-time PCR molecular antigen typing overcomes the major challenges of molecular typing by increasing laboratory productivity, reducing turnaround time, and predicting phenotypes for many antigens given the common variants for those antigens.

LinkSēq™ Portfolio for Blood Genotyping

Why Choose LinkSēq for Blood Genotyping?

More Antigens, No Chips, No Gels, No Wait

Simplicity

Fast workflow with little hands-on effort

  • 10 minutes hands-on time—simply add your DNA to the pre-aliquoted buffer/enzyme mix, add to the tray, seal, and you are done

 

Easy data analysis via SureTyper™ Blood

  • Automatically calls genotype and predicts phenotypes based on ISBT allele database and proprietary algorithm—in seconds
  • Simple data drill-down tools
  • Easy to use, intuitive interface

Confidence

Accurate real-time PCR

  • Allele-specific amplification combined with SYBR® Green to detect PCR products without chips or gels

 

Robust contamination protection

  • Samples are handled only once and then sealed in reaction trays
  • Increased sample protection as amplified PCR products remain sealed

Easy integration with data management systems via XML export

Flexibility

  • Broad instrument compatibility
  • High throughput to a single sample

Fast Turnaround

  • DNA sample to results in 90 minutes
  • Just 10 minutes of hands-on time

  

LinkSēq – Fast Workflow with Minimal Hands-on Time

90 minutes to results

  

Step 1: "Add" graphic

Add
Hands On: 10 min

  

Step 2: "Amplify" graphic

Amplify
Hands off: 70 min

  

Step 3: "Analyze" graphic

Analyze
Automated: 10 min

 


LinkSēq™ ABO Genotyping and Subgrouping 

ABO alleles encode the enzyme Glycosyltransferase. Many A subgroups involve a weakly expressed A antigen, which can cause discordant serological reactions in forward and reverse typing. Our ABO products offers a better solution to research these alleles by separating A1 from non-A1, B from B3, Bweak and Bel, and much more.

Example of results provided in our SureTyper Blood Software

SureTyper Blood Software example results for  LinkSēq™ ABO Genotyping and Subgrouping
Generate clear results in seconds
SureTyper Blood Software example results for LinkSēq™ ABO Genotyping and Subgrouping
View raw data

Coverage Includes:

  • A, B, AB, cisAB, O
  • A subgroups: A1 or non-A1, ie, A2, A3, Aweak, Ael or Am
  • B subgroups: B, B3, Bweak or Bel
  • O subgroups: O1, O2
  • Possible Bombay phenotype

LinkSēq™ Red Blood Cell (RBC) and RH Genotyping

Building complete antigen profiles via serology for the 13 key blood group systems is challenging. 

  • Serology may provide an ambiguous answer for weak and partial phenotypes. Serology is not always possible. Commercial reagents are scarce or unavailable for certain Dombrock, Colton, and Cartwright antigens, for the RhCE V and VS antigens, and more. Serology is undesirable for samples from a fetus or neonate.
  • The time and effort required to do the number of serology tests are significant if your goal is to build a complete antigen profile and samples with warm autoantibodies.

With the LinkSeq RBC and RH assays, you can genotype 13 key blood group systems plus Hemoglobin all in one run. 

Example of RBC results provided in our SureTyper Blood Software

SureTyper Blood Software example results for LinkSēq™ Red Blood Cell (RBC) and RH Genotyping
Generate clear results in seconds
SureTyper Blood Software example results for LinkSēq™ Red Blood Cell (RBC) and RH Genotyping
View raw data

Coverage Includes:
RhD, RhCE, MNS, Kell, Duffy, Kidd, Lutheran, Diego, Cartwright, Scianna, Dombrock, Colton, and Landsteiner-Wiener plus Hemoglobin n mutations S and C, and the normal A gene.

LinkSeq™ HPA Genotyping

Human Platelet Antigens (HPA) are glycoproteins expressed on platelet membranes. Our HPA assays are designed  to target both the a and b alleles for HPA-1 through HPA-11, and HPA-15.

Example of HPA Results providing in the SureTyper Blood Software

SureTyper Blood Software example results for LinkSeq™ HPA Genotyping
Generate clear results in seconds
SureTyper Blood Software example results for LinkSeq™ HPA Genotyping
View raw data

Coverage Includes:

  • HPA-1 through HPA-11
  • HPA-15
It all starts with a demo
Click here to request a demo

For Research Use Only. Not for use in diagnostic procedures.

Our Solutions

LinkSēq™ ABO Typing Kits
The LinkSēq™ platform provides ABO genotyping at a level of resolution that is higher than what is commonly used in labs.
LinkSēq™ RBC and RH Typing Kits
Fast and easy typing of red blood cell (RBC) antigens including RhD and RhCE, and Hemoglobin
LinkSēq™ HPA Typing Kits
Fast and easy genotyping of HPA1 through HPA-11 as well as HPA 15.
SureTyper™ Blood
SureTyper Blood is the software platform used with LinkSēq RBC, ABO and HPA products for automated and simplified genotype reporting.