Switch to Thermo Scientific DreamTaq DNA Polymerases for better results and added convenience.

Taq DNA Polymerase, recombinant

Catalog number:  10342020

Related applications:


| Chromatin Biology

| Methylation Analysis

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Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.

With our Taq DNA Polymerase, you get:

• Your choice of recombinant or native enzyme
• Amplification of PCR products up to 5 kb in size
• An enzyme that is licensed and qualified for PCR

Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.

Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.

Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

For Research Use Only. Not for use in diagnostic procedures.


Exonuclease Activity: 5' - 3'
Hot Start: No
Quantity: 500 units
Shipping Condition: Approved for shipment on Wet or Dry Ice
Detection Method: Primer-Probe
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Overhang: 3'-A
PCR Method: RT-PCR
Polymerase: Taq DNA Polymerase
Product Type Specs: DNA Polymerase
Reaction Format: Separate Components
Reaction Speed: Standard
Size (Final Product): 5 kb or less

Contents & storage

• 100 µl Taq DNA Polymerase (5 U/µl)
• 2.5 ml 10X PCR buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl)
• 1 ml Magnesium Chloride (50 mM)

Store at -30°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly.