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Invitrogen™

Taq DNA Polymerase, recombinant

Catalog number: 10342178
Have Questions?
Invitrogen™

Taq DNA Polymerase, recombinant

Catalog number: 10342178
Have Questions?
Catalog Number
10342178
Unit Size
5,000 units
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
100 units
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
500 units
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
3 x 500 units
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
103421785,000 units
-
10342053100 units
-
10342020500 units
-
103420463 x 500 units
-
Product Overview
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Additional Information
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Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.

With our Taq DNA Polymerase, you get:

• Your choice of recombinant or native enzyme
• Amplification of PCR products up to 5 kb in size
• An enzyme that is licensed and qualified for PCR

Applications
Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.

Source
Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.

Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Primer-probe
Format
Stand-alone enzyme
GC-Rich PCR Performance
Low
PCR Method
qPCR, Standard PCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Exonuclease Activity
5' - 3'
Product Type
DNA Polymerase
Quantity
5000 units
Shipping Condition
Approved for shipment on Wet or Dry Ice
Concentration
5 U/μL
Fidelity (vs. Taq)
1 X
Hot Start
No
No. of Reactions
5000 Reactions
Overhang
3'-A
Reaction Format
Separate Components
Size (Final Product)
5 kb or less
Starting Material
DNA

Contents & Storage

Contains:
• 1000 μl Taq DNA Polymerase (5 U/μl)
• 20 ml 10X PCR buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl)
• 10 ml Magnesium Chloride (50 mM)

Store at -30°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly.

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