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Invitrogen
CD81 Monoclonal Antibody (M38)
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FIGURE: 1 / 43
CD81 Antibody (10630D) in WB
Pharmaceutics 2022 -
Figure 2 ( A ) Description of LEV size by tunable resistive pulse sensing (TRPS). TRPS size distribution histogram of LEVs released by HUVEC cells exposed for 24 h to TNF. Bars represent the mean +- SD ( n = 2). ( B ) Characterization of LEVs using Western blotting for the presence of LEV protein markers (~1.5 x 10 8 LEVs per lane). Blot images are presented from different parts of the same membrane. ( C ) ... View More
Product Details
10630D
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M38
Immunogen
MOLT-4 (human T-ALL cell line)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient
RRID
Product Specific Information
The Exosome - anti-CD81 antibody recognizes human CD81, a member of the tetraspanin family, and is predicted to react with feline and rabbit. The antibody has been verified for western blotting of cellular and exosomal CD81 antigen, with a molecular weight of ~25 kDa, and used at 2 µg/mL under non-reducing conditions. Other applications may work but have not been tested.
Target Information
CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 26 kDa cell surface protein TAPA-1; 26-kDa cell surface protein TAPA-1; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28
Gene Aliases: CD81; CVID6; S5.7; TAPA1; TSPAN28
UniProt ID: (Human) P60033
Entrez Gene ID: (Human) 975
immunological synapse formation
adaptive immune response
immune system process
humoral immune response mediated by circulating immunoglobulin
positive regulation of inflammatory response to antigenic stimulus
myoblast fusion involved in skeletal muscle regeneration
positive regulation of B cell proliferation
receptor internalization
regulation of protein stability
macrophage fusion
CD4-positive, alpha-beta T cell costimulation
regulation of cell proliferation
positive regulation of MAPK cascade
positive regulation of transcription from RNA polymerase II promoter
viral entry into host cell
positive regulation of B cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
positive regulation of B cell activation
protein localization to lysosome
positive regulation of protein exit from endoplasmic reticulum
cellular response to low-density lipoprotein particle stimulus
protein localization to plasma membrane
osteoclast fusion
positive regulation of receptor clustering
positive regulation of protein catabolic process in the vacuole
regulation of macrophage migration
positive regulation of adaptive immune memory response
regulation of cell motility
positive regulation of T-helper 2 cell cytokine production
positive regulation of CD4-positive, alpha-beta T cell proliferation
positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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