Invitrogen™

BenchMark™ Pre-stained Protein Ladder

Catalog number:  10748010

Related applications:

Protein Electrophoresis & Western Blotting

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Description

BenchMark Pre-Stained Protein Ladder contains 10 proteins ranging in apparent molecular weight from 6 to 180 kDa for use in monitoring electrophoresis or transfer from gel to membrane in western blotting. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-PAGE gels and western blots

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Colorimetric
Gel Compatibility: Novex® Tris-Glycine Gels
Molecular Weight: 180, 115, 82, 64, 49, 37, 26, 19, 15, 6 kDa
Number of Markers: 10
Product Line: BenchMark™
Product Size: 2 x 250 µL
Ready to Load: Yes
Shipping Condition: Approved for shipment on Wet or Dry Ice
Size Range: 6 to 180 kDa
Stain Type: 2 color: Blue, Pink
System: SDS-PAGE

Contents & storage

Two vials of 250 µl each are provided in loading buffer consisting of 50 mM Tris-HCl (pH 6.8), 5 mM EDTA, 10 mM DTT, 1% (w/v) SDS, 10% (w/v) glycerol. Store at -20°C. Avoid repeated freezing and thawing.