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Sf-900™ Medium (1.3X) - FAQs

View additional product information for Sf-900™ Medium (1.3X) - FAQs (10967032)

1 product FAQs found

What medium do you recommend to grow Sf9 or Sf21 cells for performing plaque assays?

We would recommend using our Grace's Supplemented Insect Medium (Cat. No. 11667037) or SF-900 Medium (1.3X) (Cat. No. 10967032). Grace's Supplmented Insect Media is a 2X formulation intended for use in the agarose overlay when performing the plaque assay. The media has been modified with:
- Lactalbumin hydrolysate
- Yeastolate
- L-glutamine
- Sodium bicarbonate

Please go here for the complete formulation (http://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html). SF-900 Medium (1.3X) is a complete serum-free low protein insect cell culture medium for Sf9 and Sf21 cells, which can be used for 1% agarose overlays used for plaque assays.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.