Waymouth's is a chemically defined medium developed by Charity Waymouth and originally formulated for studies on the nutrition, metabolism, and growth of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Waymouth's medium is also used for the culture of whole organs and a broad range of cell types, such as carcinoma cell lines.
The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

Dual-Site cGMP Manufacturing
Gibco™ Waymouth's medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ Waymouth's product made in our Scotland facility (31220-023). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. Waymouth's contains no proteins, lipids, or growth factors. Some cell types may required the supplementation with Fetal Bovine Serum (FBS). Waymouth's uses a sodium bicarbonate buffer system (2.24 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.