VP-SFM is a serum-free, ultra-low protein (5 μg/ml) medium containing no proteins, peptides, or other components of animal or human origin. It is designed for the growth of VERO cells for virus production (1). This medium is suitable for the growth of COS-7, MDCK, BHK-21 (suspension culture) and HEp2 cells, and for recombinant protein production.

Formulated without L-glutamine for increased stability; add 20 ml of 200 mM L-glutamine or 20 ml of GlutaMAX™-I supplement per 1,000 ml of medium prior to use.