Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System
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Invitrogen™

Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System

Alternative Product: Try TaqMan Fast Virus 1-Step Master Mix, our high performance, 1-step RT-qPCR master mix. With TaqMan Fast Virus자세히 알아보기
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카탈로그 번호반응 수
11731023500 Reactions
11731015100 Reactions
카탈로그 번호 11731023
제품 가격(KRW)
6,410,000
Each
카트에 추가하기
반응 수:
500 Reactions
제품 가격(KRW)
6,410,000
Each
카트에 추가하기
Alternative Product: Try TaqMan Fast Virus 1-Step Master Mix, our high performance, 1-step RT-qPCR master mix. With TaqMan Fast Virus 1-Step Master Mix, we’ve improved on the best features of the Platinum Quantitative RT-PCR ThermoScript One-Step System and added additional convenience and performance to your 1-step gene expression analysis and RNA virus detection workflows.
Platinum Quantitative RT-PCR ThermoScript One-Step System is for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step.

This kit provides:
• Platinum™ Taq DNA Polymerase
• ThermoScript RT polymerase
• A vial of 50 mM MgSO4

For Research Use Only. Not for use in diagnostic procedures.
사양
반응 수500 Reactions
중합효소Taq DNA Polymerase
제품라인Platinum, ThermoScript
제품 유형RT-PCR One-Step System
수량500 reactions
샘플 종류RNA
배송 조건Dry Ice
충분500 Reactions
검출 방법Primer-probe
PCR 방법1-step RT-qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum™ Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO4 ). A vial of 50 mM MgSO4 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System, 500 reactions FAQs