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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.

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qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA. ROX reference dye is included in the mix for maximum convenience. These qPCR SuperMixes include:


–Platinum™ Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)

–Uracil-DNA Glycosylase (UDG): prevents carryover contamination in conjunction with dUTP (included) for improved specificity
–ROX reference dye premixed at optimal concentration for ABI instruments


qPCR SuperMixes with ROX are available in two formats:

–Platinum™ SYBR™ Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR™ Green I dye (Figure 2)
–Platinum™ qPCR SuperMix-UDG with ROX offers superior performance with either fluorogenic primers (e.g., LUX™ Primers) or probe-based detection systems (e.g., TaqMan™)


Contents and Storage:
Platinum™ SYBR™ Green qPCR SuperMix-UDG with ROX includes a 2X SuperMix with SYBR™ Green I and ROX Reference Dyes, and 50 mM MgCl_2. Platinum™ qPCR SuperMix-UDG with ROX includes a 2X SuperMix with ROX Reference Dye, and 50 mM MgCl_2. Store all components at -20°C. Guaranteed stable for 6 months when properly stored.