Platinum™ Quantitative PCR SuperMix-UDG w/ROX

Catalog number:  11743500

Invitrogen™  Related applications: Chromatin Biology | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.

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qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA. ROX reference dye is included in the mix for maximum convenience. These qPCR SuperMixes include:


–Platinum® Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)

–Uracil-DNA Glycosylase (UDG): prevents carryover contamination in conjunction with dUTP (included) for improved specificity
–ROX reference dye premixed at optimal concentration for ABI instruments


qPCR SuperMixes with ROX are available in two formats:

–Platinum® SYBR® Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR® Green I dye (Figure 2)
–Platinum® qPCR SuperMix-UDG with ROX offers superior performance with either fluorogenic primers (e.g., LUX™ Primers) or probe-based detection systems (e.g., TaqMan®)


Contents and Storage:
Platinum® SYBR® Green qPCR SuperMix-UDG with ROX includes a 2X SuperMix with SYBR® Green I and ROX Reference Dyes, and 50 mM MgCl2. Platinum® qPCR SuperMix-UDG with ROX includes a 2X SuperMix with ROX Reference Dye, and 50 mM MgCl2. Store all components at -20°C. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Product Line: Platinum®
Product Size: 500 reactions
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Dry Ice

Contents & storage

• 12.5 ml Platinum® Quantitative PCR SuperMix-UDG with ROX
• 2 × 1 ml Magnesium Chloride (50 mM)

Store at -20°C in the dark.

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Manuals & protocols

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