Platinum™ Quantitative PCR SuperMix-UDG w/ROX

Catalog number:  11743500

 Related applications: Chromatin Biology | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.

qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA. ROX reference dye is included in the mix for maximum convenience. These qPCR SuperMixes include:

–Platinum® Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)

–Uracil-DNA Glycosylase (UDG): prevents carryover contamination in conjunction with dUTP (included) for improved specificity
–ROX reference dye premixed at optimal concentration for ABI instruments

qPCR SuperMixes with ROX are available in two formats:

–Platinum® SYBR® Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR® Green I dye (Figure 2)
–Platinum® qPCR SuperMix-UDG with ROX offers superior performance with either fluorogenic primers (e.g., LUX™ Primers) or probe-based detection systems (e.g., TaqMan®)

Contents and Storage:
Platinum® SYBR® Green qPCR SuperMix-UDG with ROX includes a 2X SuperMix with SYBR® Green I and ROX Reference Dyes, and 50 mM MgCl2. Platinum® qPCR SuperMix-UDG with ROX includes a 2X SuperMix with ROX Reference Dye, and 50 mM MgCl2. Store all components at -20°C. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Product Line: Platinum®
Product Size: 500 reactions
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Dry Ice

Contents & storage

• 12.5 ml Platinum® Quantitative PCR SuperMix-UDG with ROX
• 2 × 1 ml Magnesium Chloride (50 mM)

Store at -20°C in the dark.


Manuals & protocols

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