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Invitrogen™

SuperScript™ VILO™ Master Mix

Catalog number:  11755050

Related applications:

Reverse Transcription

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Description

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR (qRT-PCR) assays.

Note: For superior cDNA synthesis performance in RT-qPCR applications, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme Function: Reverse Transcriptase
For Use With (Application): Two-step RT-qPCR
Form: Liquid
Format: Liquid
Includes: SuperScript VILO Master Mix containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, proprietary helper protein, random primers, dNTPs, MgCl&subscript_2;
No. of Reactions: 50 reactions
Optimal Reaction Temperature: 42° C
Product Line: SuperScript™, VILO™
Reverse Transcriptase: SuperScript™ III
Ribonuclease H Activity: Reduced
Shipping Condition: Dry Ice
Size (Final Product): 10 kb or less
Storage Requirements: Store in freezer (-5° to -30°C).
Template: ssRNA

Contents & storage

• SuperScript VILO Master Mix containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, proprietary helper protein, random primers, dNTPs, MgCl2

Store in freezer (-5 to -30°C).