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Invitrogen™

SuperScript™ VILO™ Master Mix

Catalog number: 11755050
Have Questions?
Invitrogen™

SuperScript™ VILO™ Master Mix

Catalog number: 11755050
Have Questions?
Catalog Number
11755050
Unit Size
50 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
250 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1175505050 reactions
-
11755500500 reactions
-
11755250250 reactions
-
Product Overview
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SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays.

Features of SuperScript VILO Master Mix include:
• Starting material can range up to 2.5 μg of total RNA in a 20 μL cDNA synthesis reaction
• Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
• RT is engineered with reduced RNase H activity and increased thermal stability

For superior cDNA synthesis performance in RT-qPCR applications
SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
qPCR
Format
Master Mix
GC-Rich PCR Performance
High
Reaction Speed
60 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
42°C
Reverse Transcriptase
SuperScript III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
For Use With (Application)
Real Time PCR (qPCR)
Concentration
5X
Final Product Type
First-Strand cDNA
No. of Reactions
50 Reactions
Reaction Format
Master Mix
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 10 kb
Starting Material
RNA

Contents & Storage

• SuperScript VILO Master Mix (200 μL)

Store at –5 to –30°C.

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