SYBR GreenER™ qPCR SuperMix Universal

Catalog number: 11762100

Invitrogen™  Related applications: Chromatin Biology | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

SYBR® GreenER™ qPCR SuperMixes Universal incorporate the latest high-performance technology to provide the most reliable gene expression data on a variety of platforms, including ABI 7500, Corbett Rotor-Gene™, MJ Chromo4™ and Opticon™, and Stratagene instruments. The SYBR® GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:
• A novel dsDNA-binding dye - exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR® Green I, while using the same instrument filters and settings
• An optimized buffer system - improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase - reduces carryover contamination in qPCR

Novel Formulation Provides More Reliable Data
The SYBR® GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range.

Consistent Specificity and Low-Copy Detection
The SYBR® GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR® GreenER™ qPCR reagent system also translates to greater sensitivity. With SYBR® GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA.

Eight cDNA targets and eight genomic DNA targets were amplified from 1 ng of cDNA and 6 pg of gDNA, respectively, on the ABI PRISM® 7900HT Sequence Detection System using the SYBR® GreenER™ qPCR SuperMix for ABI PRISM® instruments. For all 16 targets, detection was positive in the presence of template but negative for all no-template controls.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 7500 System, MJ Chromo4, Stratagene Mx3000P, MJ Opticon, Stratagene Mx3005P, Stratagene Mx4000
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Product Size: 100 reactions
Shipping Condition: Dry Ice
Sample Type (Specific): cDNA, DNA (Genomic)

Contents & storage

SYBR® GreenER™ qPCR SuperMix Universal contains:

2X SYBR® GreenER™ qPCR SuperMix and a separate tube of ROX reference dye. Store at +4°C upon receipt. Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols

Recommended products