SYBR GreenER™ qPCR SuperMix Universal

Catalog number:  11762500

 Related applications:

Chromatin Biology

| Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR GreenER qPCR SuperMix and added additional capabilities for your gene expression analysis.

SYBR® GreenER™ qPCR SuperMixes Universal incorporate the latest high-performance technology to provide the most reliable gene expression data on a variety of platforms, including ABI 7500, Corbett Rotor-Gene™, MJ Chromo4™ and Opticon™, and Stratagene instruments. The SYBR® GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:
• A novel dsDNA-binding dye - exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR® Green I, while using the same instrument filters and settings
• An optimized buffer system - improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase - reduces carryover contamination in qPCR

Novel Formulation Provides More Reliable Data
The SYBR® GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range.

Consistent Specificity and Low-Copy Detection
The SYBR® GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR® GreenER™ qPCR reagent system also translates to greater sensitivity. With SYBR® GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA.

Eight cDNA targets and eight genomic DNA targets were amplified from 1 ng of cDNA and 6 pg of gDNA, respectively, on the ABI PRISM® 7900HT Sequence Detection System using the SYBR® GreenER™ qPCR SuperMix for ABI PRISM® instruments. For all 16 targets, detection was positive in the presence of template but negative for all no-template controls.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: SYBR
For Use With (Equipment): 7500 System, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Product Line: Platinum®, SYBR® GreenER™
Product Size: 500 reactions
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Dry Ice

Contents & storage

Contains SYBR® GreenER™ qPCR SuperMix Universal (12.5 mL). SYBR® GreenER™ qPCR SuperMix Universal is a 2X SuperMix with ROX Reference dye provided in a separate tube (500 µL). Sufficient reagents are provided for 500 reactions based on a 50 µL reaction size.

Store at +4°C upon receipt.


Manuals & protocols