Gateway® BP Clonase® Enzyme Mix

Catalog number: 11789021

Invitrogen™  Related applications: Gateway Cloning

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Description

Gateway BP Clonase® enzyme contains both Int (Integrase) and IHF (Integration Host Factor) proteins that catalyze the in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and a Donor vector (containing attP sites) to generate Entry clones. BP Clonase® II enzyme format is a single mix of both enzyme and buffer to ensure enzyme stability and enable convenient 10µl reaction set up with fewer pipetting steps, which both maximize recombination efficiency and minimize pippetting. The original format of BP Clonase enzyme is the stand alone enzyme blend and reaction buffer provided in separate tubes.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme: BP Clonase
Product Size: 100 reactions
Shipping Condition: Dry Ice

Contents & storage

All Gateway® BP Clonase® enzyme kits include proteinase K solution (2 µg/µl), 30% PEG 8000/30 mM MgCl2 Solution and a positive control vector. Store BP Clonase® enzyme at -80°C; BP Clonase® II Enzyme mixtures at -20°C. Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols