Medio CD 293 (1X)
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Gibco™

Medio CD 293 (1X)

El medio CD 293 es un medio definido químicamente sin proteínas y optimizado para el crecimiento de cultivos de célulasMás información
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Número de catálogoCantidad
119130191000 mL
Número de catálogo 11913019
Precio (MXN)
-
Cantidad:
1000 mL
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El medio CD 293 es un medio definido químicamente sin proteínas y optimizado para el crecimiento de cultivos de células 293 en suspensión.El medio CD 293 no contiene proteínas ni péptidos de origen animal, vegetal o sintético.La formulación tampoco contiene lisados ni hidrolisados no definidos.

Formulado sin L-glutamina para aumentar la estabilidad; añadir 20 ml de L-glutamina 200 mM o 20 ml de suplemento GlutaMAX™-I por cada 1000 ml de medio antes de su uso.

Se ha presentado un archivo principal del medio 293 a la FDA.Si desea realizar una referencia cruzada a un archivo principal del medio CD 293, póngase en contacto con el servicio técnico o con su representante de ventas local.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de células293F, 293 (HEK)
Concentración1 X
Línea de productosGibco
Tipo de productoMedio 293
Cantidad1000 mL
Condiciones de envíoTemperatura ambiente
ClasificaciónLibre de material de origen animal, bajo en proteínas, sin suero
Culture TypeCultivos celulares en suspensión
FormularioLíquido
Serum LevelSin suero
Sin aditivosSin glutamina
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

How do I adapt my cells to serum-free medium?

Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.

SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

Why is it necessary to gradually adapt the cells to serum-free medium?

Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.