SuperScript™ Double-Stranded cDNA Synthesis Kit
SuperScript™ Double-Stranded cDNA Synthesis Kit
인용 및 참조 문헌 (1)
Invitrogen™

SuperScript™ Double-Stranded cDNA Synthesis Kit

SuperScript™ Double-Stranded cDNA Synthesis Kit는 total RNA 또는 poly A+ selected RNA (mRNA)에서 품질 우수한 double-stranded cDNA를 만드는 데 필요한자세히 알아보기
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카탈로그 번호수량
1191701010 Reactions
1191702050 Reactions
카탈로그 번호 11917010
제품 가격(KRW)
2,245,000
온라인 행사
Ends: 31-Dec-2025
2,494,000
할인액 249,000 (10%)
Each
카트에 추가하기
수량:
10 Reactions
제품 가격(KRW)
2,245,000
온라인 행사
Ends: 31-Dec-2025
2,494,000
할인액 249,000 (10%)
Each
카트에 추가하기
SuperScript™ Double-Stranded cDNA Synthesis Kit는 total RNA 또는 poly A+ selected RNA (mRNA)에서 품질 우수한 double-stranded cDNA를 만드는 데 필요한 모든 시약(oligo (dT) 함유 primer 제외)을 포함하고 있습니다. 이 kit는 SuperScript™ II reverse transcriptase의 첨단 성능과 first-strand reaction에 감소된 RNase H 활성을 사용하여 cDNA 수율이 높고 full-length cDNA를 많이 얻을 수 있습니다(1,2).
For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형cDNA (Double-Stranded)
형식Kit
라벨링 방법Direct Labeling
최적 반응 온도42°C
제품라인SuperScript
제품 유형cDNA Synthesis Kit
수량10 Reactions
역전사 효소SuperScript™ II
샘플 종류RNA
배송 조건Wet Ice
용도(애플리케이션)Microarray Analysis
Unit SizeEach
구성 및 보관
Contents: The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 μg total RNA or 0.2-5 μg mRNA into double-stranded cDNA.

Storage: Store at -20°C. All components are guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

I'd like to generate double-stranded DNA (dsDNA) from mRNA. Do you have a kit for this?

To generate double-stranded cDNA, we recommend the Maxima H Minus Double-Stranded cDNA Synthesis Kit (Cat. No. K2561) or Second Strand cDNA Synthesis Kit (Cat. No. A48570).

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Gene expression comparison of biopsies from Duchenne muscular dystrophy (DMD) and normal skeletal muscle.
Authors: Haslett Judith N.; Sanoudou Despina; Kho Alvin T.; Bennett Richard R.; Greenberg Steven A.; Kohane Isaac S.; Beggs Alan H.; Kunkel Louis M.;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12415109
'The primary cause of Duchenne muscular dystrophy (DMD) is a mutation in the dystrophin gene leading to the absence of the corresponding RNA transcript and protein. Absence of dystrophin leads to disruption of the dystrophin-associated protein complex and substantial changes in skeletal muscle pathology. Although the histological pathology of dystrophic ... More