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Invitrogen

CD66a (CEACAM1) Monoclonal Antibody (CC1), PE, eBioscience™

1 Published Figure
6 References
View all (70) CD66a (CEACAM1) antibodies

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Product Details

12-0661-80

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test
View 5 publications 5 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CC1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1311201

Product Specific Information

Description: The monoclonal antibody CC1 recognizes CD66a, also known as carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), biliary glycoprotein, and BPG. Expression of CD66a is found on brush borders, epithelial, and endothelial cells. In hematopoietic cells expression is found abundantly on B cells, as well as some NKs, monocytes, DCs, and granulocytes. Although low levels of mRNA have been identified in T cells in humans, resting mouse T lymphocytes are not reported to express CD66a, as confirmed by lack of staining with CC1 antibody. In humans, expression levels of CD66a have been used to identify malignancies. CD66a plays a key role as a regulator of BCR activation of B lymphocytes.

An alternate allele, CEACAM1b, is expressed in SJL mice; therefore, CC1 does not stain SJL tissue. The monoclonal CC1 has been shown to block viral infection and also enhance B cell proliferation when combined with IgM crosslinking.

Applications Reported: This CC1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CC1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CEACAM1 is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: BGP-1; biliary glycoprotein 1; biliary glycoprotein D; carcinoembryonic antigen 1; carcinoembryonic antigen 7; carcinoembryonic antigen-related cell adhesion molecule 1; CEA-related cell adhesion molecule 1; hepatitis virus (MHV-4) susceptibility; hepatitis virus receptor; MHV-R; murine hepatitis virus receptor; unnamed protein product

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Gene Aliases: bb-1; Bgp; Bgp1; C-CAM; Cc1; CD66a; Cea-1; Cea-7; Cea1; Cea7; Hv-2; Hv2; mCEA1; Mhv-1; MHVR; MHVR1; mmCGM1; mmCGM1a; mmCGM2

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UniProt ID: (Mouse) P31809

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Entrez Gene ID: (Mouse) 26365

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Function(s)
actin binding granulocyte colony-stimulating factor receptor binding protein binding calmodulin binding bile acid transmembrane transporter activity kinase binding protein kinase binding protein phosphatase binding filamin binding Toll-like receptor binding identical protein binding protein homodimerization activity protein dimerization activity protein tyrosine kinase binding
Process(es)
regulation of cell growth blood vessel development negative regulation of cytokine production negative regulation of T cell mediated cytotoxicity regulation of immune system process negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target negative regulation of protein kinase activity cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules signal transduction negative regulation of cell proliferation regulation of endothelial cell migration bile acid and bile salt transport regulation of cell migration negative regulation of granulocyte differentiation negative regulation of interleukin-1 production negative regulation of interleukin-2 production cellular response to insulin stimulus common myeloid progenitor cell proliferation insulin receptor internalization granulocyte colony-stimulating factor signaling pathway regulation of epidermal growth factor receptor signaling pathway negative regulation of T cell proliferation negative regulation of vascular permeability negative regulation of cytotoxic T cell degranulation positive regulation of MAP kinase activity wound healing, spreading of cells cell-cell junction organization regulation of endothelial cell differentiation negative regulation of osteoclast differentiation negative regulation of fatty acid biosynthetic process negative regulation of bone resorption negative regulation of JNK cascade positive regulation of JNK cascade negative regulation of T cell receptor signaling pathway negative regulation of lipid biosynthetic process regulation of protein kinase B signaling regulation of blood vessel remodeling regulation of ERK1 and ERK2 cascade negative regulation of platelet aggregation cell-cell adhesion insulin catabolic process regulation of homophilic cell adhesion positive regulation of homophilic cell adhesion regulation of sprouting angiogenesis negative regulation of hepatocyte proliferation positive regulation of vasculogenesis
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