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Invitrogen

Phospho-PLCG2 (Tyr759) Monoclonal Antibody (4NPRN4), PE, eBioscience™

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Additional Information:

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Product Details

12-9866-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4NPRN4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2723469

Product Specific Information

Applications Reported: This 4NPRN4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4NPRN4 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC gamma1, PLC gamma2, PLC delta1, PLC delta2 and PLCe. PLC delta exists as four different isoforms. PLC delta1, a calcium signal amplifier, is activated by an atypical GTP-binding protein. In addition, PLC delta1 is an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and alpha1B-adrenoreceptor signaling. Mouse PLC delta1 is highly expressed in brain, heart, lung and testis. PLC delta is abnormally accumulated in autopsied brains with Alzheimer's disease (AD), suggesting that it may play a role in the pathology of AD. PLC delta2 is markedly expressed in type II intestinal metaplasia and in the adenocarcinoma. When PLC delta2 is expressed in type I intestinal metaplasia, the metaplasia is generally considered benignant, yet evolves toward neoplastic transformation. Thus, PLC delta2 expression may be a possible marker of gastric malignant transformation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: phosphoinositide phospholipase C-gamma-2; phospholipase C; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C-IV; PLC gamma 2; PLC gamma-2; PLC gamma2; PLC- gamma2; PLC-gamma; unnamed protein product

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Gene Aliases: APLAID; FCAS3; PLC-gamma-2; PLC-IV; Plcg-2; PLCgamma2

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UniProt ID: (Human) P16885, (Mouse) Q8CIH5

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Entrez Gene ID: (Human) 5336, (Mouse) 234779

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Function(s)
phosphotyrosine binding phosphatidylinositol phospholipase C activity phospholipase C activity protein binding phosphoric diester hydrolase activity protein kinase binding scaffold protein binding phosphorylation-dependent protein binding protein tyrosine kinase binding phosphatidylcholine phospholipase C activity
Process(es)
cell activation response to yeast positive regulation of receptor internalization stimulatory C-type lectin receptor signaling pathway toll-like receptor signaling pathway macrophage activation involved in immune response follicular B cell differentiation positive regulation of dendritic cell cytokine production lipid metabolic process phosphatidylinositol biosynthetic process signal transduction positive regulation of cytosolic calcium ion concentration phospholipid catabolic process regulation of gene expression positive regulation of gene expression positive regulation of epithelial cell migration regulation of lipid metabolic process calcium-mediated signaling B cell differentiation lipopolysaccharide-mediated signaling pathway response to magnesium ion activation of store-operated calcium channel activity positive regulation of type I interferon production response to lipopolysaccharide positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-2 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production inositol trisphosphate biosynthetic process response to ATP intracellular signal transduction B cell activation negative regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade phosphatidylinositol metabolic process phosphatidylinositol-mediated signaling response to axon injury positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway release of sequestered calcium ion into cytosol positive regulation of phagocytosis, engulfment positive regulation of macrophage cytokine production antifungal innate immune response regulation of calcineurin-NFAT signaling cascade cellular response to calcium ion cellular response to lipid positive regulation of neuroinflammatory response positive regulation of NLRP3 inflammasome complex assembly positive regulation of intracellular signal transduction positive regulation of cell cycle G1/S phase transition positive regulation of reactive oxygen species biosynthetic process cellular response to lectin Wnt signaling pathway platelet activation Fc-epsilon receptor signaling pathway antigen receptor-mediated signaling pathway
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