PureLink™ RNA Micro Scale Kit

PureLink™ RNA Micro Scale Kit - FAQs

2 product FAQs found

I am using the PureLink RNA Micro Kit, and my RNA yield is very low. Can you offer some tips?

This could be due to incomplete lysis and homogenization. Here are some recommendations:

- Ensure that 10 µL of 2-mercaptoethanol was added per 1 mL of Lysis Buffer.
- Perform all steps at room temperature unless directed otherwise.
- Decrease the amount of starting material used, or increase volume of Lysis Buffer.
- Use the proper homogenization methods according to recommendations in the sample-specific protocols.
- Cut tissue samples into smaller pieces and ensure the tissue is completely immersed in the Lysis Buffer to achieve optimal lysis.

Are the buffers (Lysis Buffer, Wash Buffer I, and Wash Buffer II) used within the PureLink RNA Mini Kit (Cat. No. 12183025) and the PureLink RNA Micro Scale Kit (Cat. No. 12183016) the same?

Yes, the compositions for Lysis Buffer, Wash Buffer I, and Wash Buffer II are the same between the PureLink RNA Mini Kit (Cat. No. 12183025) and the PureLink RNA Micro Scale Kit (Cat. No. 12183016).