ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR. This dye has been qualified for use on the ABI PRISM™ 7700 with Platinum™ Quantitative PCR SuperMix-UDG™ , Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript™ III Platinum™ One-Step qRT-PCR Kit. ROX Reference Dye:
Normalizes non-PCR-related fluctuations in fluorescence
• Provides a stable baseline for multiplex quantitative PCR and RT-PCR
• Uses existing ROX spectral settings, eliminating the need to re-calibrate the thermal cycler
For Research Use Only. Not for use in diagnostic procedures.