Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™221 vector has a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
For Research Use Only. Not for use in diagnostic procedures.
Gateway System Destination Expression Vector
Contents & Storage
6 µg pDONR221™ vector, lyophilized in TE buffer, pH 8.0. Upon receipt, store at -20°C.
Figure 1 - Directional cloning of PCR products with Gateway® Technology