MultiSite Gateway® Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway® destination vector

Catalog number: 12537100

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


MultiSite Gateway® Pro Technology enables you to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation into a Gateway® Expression vector. Using specifically designed att sites for recombinational cloning, you can clone two, three, or four DNA fragments into any Gateway® Destination vector containing attR1 and attR2 sites. The resulting expression clone is ready for downstream expression and analysis applications. The new MultiSite Gateway® Pro Technology allows you to:

• Clone multiple DNA fragments into one vector without using restriction enzymes or ligases
• Take full advantage of the wide selection of Gateway® Destination vectors (available from Invitrogen) or create your own
• Replace multiple plasmid transfections with a single vector carrying all DNA elements of interest

MultiSite Gateway® Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway® Destination vector. All of the kits include internal positive control entry clones for troubleshooting potential entry clone issues.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: None (Promoterless)
Vector Type: MultiSite Gateway Vectors
Key Function: Multi-Gene Expression, Clone your own Promoter
Product Size: 20 reactions
Cloning Method: Gateway®
Number of Fragments: Up to 4 Fragments

Contents & storage

The MultiSite Gateway® Pro Kits include vectors and primers as indicated in Table 1, One Shot® Mach1™ T1R Chemically Competent Cells, and LR Clonase® II Plus and BP Clonase® II enzymes. Store cells at -80°C. Store Clonase® enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols

Recommended products