MultiSite Gateway® Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway® destination vector

Catalog number: 12537100

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

MultiSite Gateway® Pro Technology enables you to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation into a Gateway® Expression vector. Using specifically designed att sites for recombinational cloning, you can clone two, three, or four DNA fragments into any Gateway® Destination vector containing attR1 and attR2 sites. The resulting expression clone is ready for downstream expression and analysis applications. The new MultiSite Gateway® Pro Technology allows you to:


• Clone multiple DNA fragments into one vector without using restriction enzymes or ligases
• Take full advantage of the wide selection of Gateway® Destination vectors (available from Invitrogen) or create your own
• Replace multiple plasmid transfections with a single vector carrying all DNA elements of interest


MultiSite Gateway® Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway® Destination vector. All of the kits include internal positive control entry clones for troubleshooting potential entry clone issues.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cloning Method: Gateway®
Key Function: Clone your own Promoter, Multi-Gene Expression
Number of Fragments: Up to 4 Fragments
Product Size: 20 reactions
Promoter: None (Promoterless)
Vector Type: MultiSite Gateway Vectors

Contents & storage

The MultiSite Gateway® Pro Kits include vectors and primers as indicated in Table 1, One Shot® Mach1™ T1R Chemically Competent Cells, and LR Clonase® II Plus and BP Clonase® II enzymes. Store cells at -80°C. Store Clonase® enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

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