MultiSite Gateway® Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway® destination vector

Catalog number: 12537100

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

MultiSite Gateway® Pro Technology enables you to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation into a Gateway® Expression vector. Using specifically designed att sites for recombinational cloning, you can clone two, three, or four DNA fragments into any Gateway® Destination vector containing attR1 and attR2 sites. The resulting expression clone is ready for downstream expression and analysis applications. The new MultiSite Gateway® Pro Technology allows you to:


• Clone multiple DNA fragments into one vector without using restriction enzymes or ligases
• Take full advantage of the wide selection of Gateway® Destination vectors (available from Invitrogen) or create your own
• Replace multiple plasmid transfections with a single vector carrying all DNA elements of interest


MultiSite Gateway® Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway® Destination vector. All of the kits include internal positive control entry clones for troubleshooting potential entry clone issues.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: None (Promoterless)
Vector Type: MultiSite Gateway Vectors
Key Function: Multi-Gene Expression, Clone your own Promoter
Product Size: 20 reactions
Cloning Method: Gateway®
Number of Fragments: Up to 4 Fragments

Contents & storage

The MultiSite Gateway® Pro Kits include vectors and primers as indicated in Table 1, One Shot® Mach1™ T1R Chemically Competent Cells, and LR Clonase® II Plus and BP Clonase® II enzymes. Store cells at -80°C. Store Clonase® enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

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