MultiSite Gateway™ Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway™ destination vector
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引用資料與參考文獻 (2)
Invitrogen™

MultiSite Gateway™ Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway™ destination vector

MultiSite Gateway™ Pro Technology enables you to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation深入閱讀
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產品號碼Quantity
1253710020 reactions
產品號碼 12537100
價格 (HKD)
38,949.00
Each
新增至購物車
Quantity:
20 reactions
價格 (HKD)
38,949.00
Each
新增至購物車
MultiSite Gateway™ Pro Technology enables you to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation into a Gateway™ Expression vector. Using specifically designed att sites for recombinational cloning, you can clone two, three, or four DNA fragments into any Gateway™ Destination vector containing attR1 and attR2 sites. The resulting expression clone is ready for downstream expression and analysis applications. The new MultiSite Gateway™ Pro Technology allows you to:


• Clone multiple DNA fragments into one vector without using restriction enzymes or ligases
• Take full advantage of the wide selection of Gateway™ Destination vectors (available from Invitrogen) or create your own
• Replace multiple plasmid transfections with a single vector carrying all DNA elements of interest


MultiSite Gateway™ Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway™ Destination vector. All of the kits include internal positive control entry clones for troubleshooting potential entry clone issues.
For Research Use Only. Not for use in diagnostic procedures.
規格
For Use With (Application)Clone your own Promoter, Multi-Gene Expression
Product TypeGateway System Destination Expression Vector
Quantity20 reactions
VectorMultiSite Gateway Vectors
Cloning MethodGateway
Product LineGateway
PromoterNone (Promoterless)
Unit SizeEach
內容物與存放
The MultiSite Gateway™ Pro Kits include vectors and primers as indicated in Table 1, One Shot™ Mach1™ T1R Chemically Competent Cells, and LR Clonase™ II Plus and BP Clonase™ II enzymes. Store cells at -80°C. Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

常見問答集 (常見問題)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all MultiSite Gateway™ Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway™ destination vector FAQs

引用資料與參考文獻 (2)

引用資料與參考文獻
Abstract
The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs.
Authors:Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, Yost HJ, Kanki JP, Chien CB,
Journal:Dev Dyn
PubMed ID:17937395
'Transgenesis is an important tool for assessing gene function. In zebrafish, transgenesis has suffered from three problems: the labor of building complex expression constructs using conventional subcloning; low transgenesis efficiency, leading to mosaicism in transient transgenics and infrequent germline incorporation; and difficulty in identifying germline integrations unless using a fluorescent ... More
Building blocks for plant gene assembly.
Authors:Karimi M, Bleys A, Vanderhaeghen R, Hilson P,
Journal:Plant Physiol
PubMed ID:17965171
The MultiSite Gateway cloning system, based on site-specific recombination, enables the assembly of multiple DNA fragments in predefined order, orientation, and frame register. To streamline the construction of recombinant genes for functional analysis in plants, we have built a collection of 36 reference Gateway entry clones carrying promoters, terminators, and ... More