Gibco™

TrypLE™ Select Enzyme (1X), no phenol red

Green features
Catalog number: 12563029
Gibco™

TrypLE™ Select Enzyme (1X), no phenol red

Green features
Catalog number: 12563029
Catalog Number
12563029
also known as 12563-029
Unit Size
500 mL
Price (USD)
135.00
Your Price:
Availability
***
Quantity
Catalog Number
12563011
also known as 12563-011
Unit Size
100 mL
Price (USD)
41.06
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12563029
also known as 12563-029
500 mL
135.00
Your Price:
***
12563011
also known as 12563-011
100 mL
41.06
Your Price:
***
Product Overview
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Additional Information
Recommendations
TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Select is:

Gentle on cells—maintains cell health for reproducible results
Room-temperature stable—convenient to use
Easy to use—substitutes directly into existing protocols, with no inactivation required
Animal origin free (AOF)—pure, consistent, and effective

This TrypLE™ Select is modified as follows:

WithWithout

• EDTA

• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

Product Intended Use
Customers using Gibco® TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Source
Animal origin-free
Classification
Animal Origin-Free
pH
7 to 7.4
Concentration
1 X
Product Type
Cell Culture Dissociation Reagents
Chelators
EDTA
Endotoxin Level
Low
Enzyme Stability
Room Temperature Stable
pH Range
7.0 - 7.4
Osmolality
280 - 300 mOsm/kg
Shipping Condition
Room Temperature
Tested For
In Vitro Bioassay
Volume (Metric)
500 ml
Product Line
Gibco™
Form
Liquid
Phenol Red Indicator
No Phenol Red
Shelf Life
24 Months
Green Features
Energy efficient, Sustainable packaging
Quantity
500mL
Format
Bottle(s)
Reagent Type
Trypsin Replacement
Cell Type
Mammalian
Culture Type
Adherent Cell Culture

Contents & Storage

Storage conditions: 15–30°C, protect from light.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.

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Frequently asked questions (FAQs)

Citations & References