TrypLE™ Select Enzyme (1X), no phenol red, 500 mL - FAQs

View additional product information for TrypLE™ Select Enzyme (1X), no phenol red - FAQs (12563029, 12563011, 12563030, 12563031)

16 product FAQs found

Can I use TrypLE Select Enzyme to detach cells from glass surfaces?

Yes, TrypLE Select Enzyme can detach cells from glass surfaces.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the stability of TrypLE Select Enzyme (1X), no phenol red, (Cat. Nos. 12563029, 12563011) at 15-30 degrees C, 2-8 degrees C, and -5 to -20 degrees C?

The shelf life of TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563029, 12563011) is 24 months from the date of the manufacturer when stored at 15-30 degrees C, or 2-8 degrees C, and protected from light.

It can be stored at -5 to -20 degrees C for at least 22 months without affecting its performance.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563029, 12563011) sensitive to light?

We do not have specific data on the light sensitivity of TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563029, 12563011). However, the protease generated by the confidential organism has been observed to be less effective when exposed to constant light. Therefore, we recommend storing the product away from light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can you provide a statement regarding the customer’s responsibility to ensure the elimination of a reagent, such as the TrypLE Select Enzyme, from their drug product and determination of drug product safety?

The following statement is regarding the customer’s responsibility to ensure the elimination of a reagent, such as the TrypLE Select Enzyme (1X), no phenol red (Cat. Nos. 12563011, 12563029), from their drug product and determination of drug product safety:

"Products are not manufactured by Thermo Fisher under a pharmaceutical quality management system to excipient requirements and are not intended to be used as excipients. Customers are responsible for properly qualifying any products it intends to use in or as excipients – this may include steps such as washing, filtration, and sterilization of these products."

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What enzymes do you recommend for passaging mesenchymal stem cells (MSC)?

We recommend using TrypLE Express Enzyme or TrypLE Select Enzyme for passaging mesenchymal stem cells (MSCs). The usage of trypsin will result in lower cell viability upon passage. TrypLE Express and TrypLE Select are defined, animal origin-free, and stable at both room and refrigerated temperatures.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of TrypLE Select Enzyme (1X) (Cat. No. 12563029, 12563011)?

TrypLE Select Enzyme has a shelf life of 24 months from the date of manufacture, when stored as recommended (15-30 degrees C).

Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?

There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For your TrypLE products, how does the enzyme get inactivated?

Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:

After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why are my cells not adhering when I transfer them to a new flask?

Trypsinize this type of cells for a shorter duration or use less trypsin or Gibco TrypLE Select or Gibco TrypLE Express Trypsin Replacement. After trypsinization dilute in 2 to 5 mL of cell culture growth medium transfer the cell suspension to 15 mL centrifuge tube. Centrifuge for 5-10 minutes at 100 x g. Discard the supernatant and suspend cell pellet in 2-5 mL fresh medium. Determine the count and seed the flask according to normal protocol and cell should adhere to the surface.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you have a non-animal-origin enzyme for cell dissociation?

Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What can be used to detach cells from plates when coated with a thin layer of collagen I?

Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.

Find additional tips, troubleshooting help, and resources within our Extracellular Matrices (ECM) Support Center.