SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
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SuperScript&trade; III One-Step RT-PCR System with Platinum&trade; <i>Taq</i> DNA Polymerase
인용 및 참조 문헌 (3)
Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase는 end point detection에 최고의 민감도를 제공합니다. 이 시스템은 SuperScript™ III자세히 알아보기
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카탈로그 번호반응 수
1257401825 Reactions
12574026100 Reactions
카탈로그 번호 12574018
제품 가격(KRW)
407,000
Each
카트에 추가하기
반응 수:
25 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
407,000
Each
카트에 추가하기
SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase는 end point detection에 최고의 민감도를 제공합니다. 이 시스템은 SuperScript™ III RT의 높은 열안정성과 Platinum™ Taq DNA Polymerase의 특이성을 모두 갖추어 priming 특이성과 수율을 높이고 넓은 범위의 표적을 파악합니다. one-step format으로 유전자 발현 분석, 드문 바이러스 RNA 검출을 쉽게 할 수 있습니다.

• 민감도 – 대개 총 RNA 0.01 pg까지 검출(그림 1)
• 편의성 – One-step format으로 빠르고 편리하며 반응 간 변동성을 줄입니다.
• 특이성 – 최대 55°C에서 cDNA 합성에 SuperScript™ III RT를 사용해 유전자 특정 프라이머의 priming 특이성을 높입니다(그림 2)
• Amplicon 크기 – 표적 길이 4.5 kb까지 검출하여 활용도가 높습니다.

이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형PCR Amplified cDNA
형식One-Step RT-PCR System
핫 스타트Built-In Hot Start
반응 수25 Reactions
최적 반응 온도50°C
중합효소Platinum Taq DNA Polymerase
수량25 rxns
반응 형식Premixed Components
시약 유형Reverse Transcription
역전사 효소SuperScript III
리보뉴클레아제 H 활성Reduced
배송 조건Dry Ice
크기(최종 제품)Up to 4.5 kb
시작 물질RNA
기술1-Step RT-PCR
검출 방법Gel Electrophoresis
GC-Rich PCR PerformanceHigh
PCR 방법1-step RT-PCR
반응 속도30 min.
Unit SizeEach
구성 및 보관

• SuperScript III RT/Platinum Taq Mix, 50 μL
• 2X Reaction Mix, 1 mL
• Magnesium Sulfate, 500 μL (5 mM)

Store at –10 to –30°C.

자주 묻는 질문(FAQ)

How can I remove genomic DNA contamination from my sample prior to performing RT-PCR?

We recommend using ezDNase (Cat. No. 11766051). ezDNase Enzyme's high specificity for double-stranded DNA enables efficient and fast genomic DNA removal without reduction in the quality or quantity of RNA. ezDNase Enzyme is heat-labile and so can be easily deactivated by heat treatment at moderate temperature (55 degrees C). These features make ezDNase Enzyme an excellent choice for genomic DNA removal prior to reverse transcription reactions.

How much RNA should be employed for first-strand cDNA synthesis?

The amount of RNA template for a cDNA synthesis is highly flexible and depends upon the amount of sample available and an individual's need. In general, 1 µg total RNA is used in a typical 20-µL RT reaction.

Find additional tips, troubleshooting help, and resources within ourReverse Transcription and RACE Support Center.

Should I treat the cDNA with RNase H prior to downstream processing?

RNase H treatment is not always necessary. Many PCR reactions work without it. However, for cDNA synthesized with RNase H-deficient reverse transcriptases (like SuperScript II, III, and IV), RNA/cDNA hybrids—especially GC-rich ones—may not denature well, reducing PCR sensitivity. RNase H treatment can help in such cases. Additionally, RNase H treatment is beneficial for cloning larger fragments.

What percentage of RNA is converted to cDNA when performing reverse transcription?

This depends highly on the quality of the sample. mRNA itself makes up 1-5% of total RNA. Depending on the primer and enzyme used, reverse transcription can covert >70% of that into cDNA.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

I'm setting up my RT reaction and am trying to decide whether I should use random primers, oligo(dT) primer, gene-specific primer, or oligo(dT)/random mix primers. What would you suggest?

Random primers are the best choice for degraded RNA, RNA with heavy secondary structure, non-polyadenylated RNA, or prokaryotic RNA. It is recommended only for two-step RT-PCR, and typically gives the highest yields, although the cDNA may not necessarily be full length. Oligo(dT) primers are good to use when trying to recover full-length cDNA from 2-step RT-PCR. The reaction is influenced by secondary structure and RNA quality. Gene specific primers should be used for very specific, mainly one-step RT-PCR reactions.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

View all SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase, 25 rxns FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Clinical accuracy of a PLEX-ID flu device for simultaneous detection and identification of influenza viruses A and B.
Authors:Tang YW, Lowery KS, Valsamakis A, Schaefer VC, Chappell JD, White-Abell J, Quinn CD, Li H, Washington CA, Cromwell J, Giamanco CM, Forman M, Holden J, Rothman RE, Parker ML, Ortenberg EV, Zhang L, Lin YL, Gaydos CA,
Journal:J Clin Microbiol
PubMed ID:23077123
Respiratory tract infections caused by influenza A and B viruses often present nonspecifically, and a rapid, high-throughput laboratory technique that can identify influenza viruses is clinically and epidemiologically desirable. The PLEX-ID Flu assay (Abbott Molecular Inc., Des Plaines, IL) incorporates multilocus PCR and electrospray ionization-mass spectrometry to detect and differentiate ... More
Identification of a novel coronavirus in patients with severe acute respiratory syndrome.
Authors:Drosten C, Günther S, Preiser W, van der Werf S, Brodt HR, Becker S, Rabenau H, Panning M, Kolesnikova L, Fouchier RA, Berger A, Burguière AM, Cinatl J, Eickmann M, Escriou N, Grywna K, Kramme S, Manuguerra JC, Müller S, Rickerts V, Stürmer M, Vieth S, Klenk HD, Osterhaus AD, Schmitz H, Doerr HW,
Journal:N Engl J Med
PubMed ID:12690091
'BACKGROUND: The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent. METHODS: Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques. RESULTS: A ... More
Two isoforms of Npap60 (Nup50) differentially regulate nuclear protein import.
Authors:Ogawa Y, Miyamoto Y, Asally M, Oka M, Yasuda Y, Yoneda Y,
Journal:Mol Biol Cell
PubMed ID:20016008
Npap60 (Nup50) is a nucleoporin that binds directly to importin alpha. In humans, there are two Npap60 isoforms: the long (Npap60L) and short (Npap60S) forms. In this study, we provide both in vitro and in vivo evidence that Npap60L and Npap60S function differently in nuclear protein import. In vitro binding ... More