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Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 12574018
Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 12574018
Catalog Number
12574018
also known as 12574-018
Unit Size
25 reactions
Price (USD)
249.00
Your Price:
Availability
***
Quantity
Catalog Number
12574026
also known as 12574-026
Unit Size
100 reactions
Price (USD)
772.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12574018
also known as 12574-018
25 reactions
249.00
Your Price:
***
12574026
also known as 12574-026
100 reactions
772.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers, and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript® III Reverse Transcriptase and Platinum® Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 µg of total RNA. Key advantages of this kit:

Sensitive—routine detection down to 0.01 pg total RNA (see figure)
Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
Specificity—uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers (see figure)
Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility

SuperScript® III Reverse Transcriptase
SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.

Platinum® Taq DNA polymerase
Platinum® Taq DNA polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
RT-PCR
Includes
50μL SuperScript III RT/Platinum Taq Mix; 1mL 2X Reaction Mix (containing 0.4mM of each dNTP, 3.2mM MgSO4); 500μL 5mM Magnesium Sulfate
No. of Tests
25 Reactions
Primer Probe Compatibility
Unlabeled Oligos
Product Line
Platinum™, SuperScript™
Product Type
One-Step RT-PCR System
Quantity
25 reactions
Reverse Transcriptase
SuperScript™ III
Shipping Condition
Dry Ice
Sufficient For
25 Reactions
Detection Method
Gel Electrophoresis
Format
Tube
GC-Rich PCR Performance
High
Hot Start
Built-In Hot Start
No. of Reactions
25 Reactions
PCR Method
1-Step RT-PCR
Polymerase
Platinum™ Taq DNA Polymerase
Reaction Speed
Fast
Sample Type
RNA
Size (Final Product)
4.5 kb or less
Volume
50 μL

Contents & Storage

Components:
• 50 µL SuperScript™ III RT/Platinum™ Taq Mix
• 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 3.2 mM MgSO4)
• 500 µL 5 mM Magnesium Sulfate

Store all components at -30°C to -10°C.

Figures

Frequently asked questions (FAQs)

Citations & References