Informational:For superior RT-PCR performance, upgrade to the new SuperScript IV One-Step RT-PCR System.
Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 12574018
Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number: 12574018
Catalog Number
12574018
Unit Size
25 rxns
Price (USD)
Price: 272.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 rxns
Price (USD)
Price: 830.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1257401825 rxns
Price: 272.00
Your Price:
***
12574026100 rxns
Price: 830.00
Your Price:
***
Product Overview
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The SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers, and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 µg of total RNA. Key advantages of this kit:

Sensitive—routine detection down to 0.01 pg total RNA (see figure)
Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
Specificity—uses SuperScript™ III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers (see figure)
Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility

SuperScript™ III Reverse Transcriptase
SuperScript™ III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.

Platinum™ Taq DNA polymerase
Platinum™ Taq DNA polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Gel Electrophoresis
Format
Kit
GC-Rich PCR Performance
High
Hot Start
Built-In Hot Start
No. of Reactions
25 Reactions
PCR Method
1-Step RT-PCR
Polymerase
Platinum Taq
Reaction Format
Master Mix
Reaction Speed
Standard
Reagent Type
Reverse Transcription
Sample Type
RNA
Size (Final Product)
4.5 kb or Less
Starting Material
RNA
Technique
1-Step RT-PCR
Volume
50 μL
Includes
50μL SuperScript III RT/Platinum Taq Mix; 1mL 2X Reaction Mix (containing 0.4mM of each dNTP, 3.2mM MgSO4); 500μL 5mM Magnesium Sulfate
No. of Tests
25 Reactions
Primer Probe Compatibility
Unlabeled Oligos
Product Line
Platinum™, SuperScript™
Product Type
One-Step RT-PCR System
Quantity
25 Reactions
Reverse Transcriptase
SuperScript™ III
Shipping Condition
Dry Ice
Sufficient For
25 Reactions
For Use With (Application)
RT-PCR

Contents & Storage

Components:
• 50 μL SuperScript™ III RT/Platinum™ Taq Mix
• 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 3.2 mM MgSO4)
• 500 μL 5 mM Magnesium Sulfate

Store all components at -30°C to -10°C.