Invitrogen™

Champion™ pET160 Gateway™ Expression Kit with Lumio™ Technology

Catalog number:  12583035

Related applications:

Gateway Cloning

| Protein Assays and Analysis

| Protein Expression

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Description

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Bacterial or Yeast Strain: BL21 Star™(DE3), TOP10
Cleavage: TEV Protease Recognition Site
Cloning Method: Gateway®
Detection Location: In-Gel Detection
Expression Mechanism: Cell-Based Expression
Expression System: E. coli
Product Line:
Constitutive or Inducible System: Inducible
Inducing Agent: IPTG
Promoter: T7
Selection Agent (Eukaryotic): None
Vector: pET
Protein Tag: His Tag (6x), Lumio

Contents & storage

All destination vectors are provided lyophilized and supercoiled.