Champion™ pET160 Gateway® Expression Kit with Lumio™ Technology

Catalog number: 12583035

Invitrogen™  Related applications: Gateway Cloning | Protein Assays and Analysis | Protein Expression

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To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.


Expression: E. coli
Promoter: T7
Bacterial or Yeast Strain: TOP10, BL21 Star™(DE3)
Cleavage: TEV Protease Recognition Site
Vector Type: pET
Product Size: 6 µg
Cloning Method: Gateway®
Inducing Agent: IPTG
Detection Location: In-Gel Detection
Expression Mechanism: Cell-Based Expression
Protein Tag or Fusion: His Tag (6x), Lumio
Selection Agent (Eukaryotic): None
Constitutive or Inducible System: Inducible
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)

Contents & storage

All destination vectors are provided lyophilized and supercoiled.


Manuals & protocols