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Invitrogen

CD45RB Monoclonal Antibody (C363.16A), Biotin, eBioscience™

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View all (46) CD45RB antibodies

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Product Details

13-0455-85

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.125 µg/test
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Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C363.16A

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Amount

500 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466460

Product Specific Information

Description: The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45. This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.

Applications Reported: The C363.16A antibody has been reported for use in flow cytometric analysis.

Applications Tested: The C363.16A antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 200 kDa T-cell leukocyte common antigen isoform; CD45; Ly5; glycoprotein isoform Ly-5; leukocyte common antigen; lymphocyte antigen 5; lymphocyte common antigen; T200 glycoprotein; unnamed protein product

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)
protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity receptor binding protein binding heparin binding protein kinase binding protein tyrosine kinase inhibitor activity ankyrin binding spectrin binding identical protein binding heparan sulfate proteoglycan binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter natural killer cell differentiation negative regulation of T cell mediated cytotoxicity positive regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation positive regulation of immunoglobulin production positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration leukocyte cell-cell adhesion signal transduction response to gamma radiation regulation of gene expression dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation negative regulation of protein autophosphorylation regulation of interleukin-8 production negative regulation of interleukin-2 production positive regulation of interleukin-2 production positive regulation of tumor necrosis factor production heterotypic cell-cell adhesion T cell proliferation B cell proliferation positive regulation of T cell proliferation positive regulation of MAP kinase activity positive regulation of MAPK cascade cell cycle phase transition plasma membrane raft distribution positive thymic T cell selection negative thymic T cell selection positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation negative regulation of JAK-STAT cascade positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes bone marrow development stem cell development negative regulation of peptidyl-tyrosine phosphorylation regulation of phagocytosis positive regulation of phagocytosis positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway regulation of T cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of Fc receptor mediated stimulatory signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade negative regulation of interleukin-4-mediated signaling pathway positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
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