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Invitrogen

Connexin 43 Monoclonal Antibody (Connexin43 (1A)), Biotin, eBioscience™

View all (42) Connexin 43 antibodies

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Product Details

13-4759-82

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL
-

Immunocytochemistry (ICC/IF)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Connexin43 (1A)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572790

Product Specific Information

Description: The Connexin43 monoclonal antibody recognizes mouse Connexin 43. Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication. Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins. Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties. Connexin 43 is expressed in many tissues, including the central nervous system, heart, and bone. Connexin 43 is the most abundant connexin found in the myocardium and is also the primary connexin expressed in astrocytes. Mutations in connexin 43 are associated with ocluodentodigital dysplasia, a rare developmental disorder characterized by craniofacial and limb abnormalities. Connexin 43 may also be a promising therapeutic target in the treatment of malignant melanomas and mammary gland tumors.

The monoclonal antibody Connexin43 also recognizes human and rat connexin 43.

Applications Reported: This Connexin43 antibody has been reported for use in immunocytochemistry, and immunohistochemical staining of frozen tissue sections.

Applications Tested: This Connexin43 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 1 connexin; connexin 43; connexin k1; connexin-43; Cx-43; CXA1; DFNB38; gap junction 43 kDa heart protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; GJA1; Npm1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product; VEGF

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Gene Aliases: AVSD3; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxnk1; EKVP; EKVP3; Gja-1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

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UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Human) 2697, (Mouse) 14609, (Rat) 24392

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Function(s)
receptor binding gap junction channel activity protein binding beta-catenin binding ion transmembrane transporter activity efflux transmembrane transporter activity tubulin binding transmembrane transporter activity glutathione transmembrane transporter activity alpha-tubulin binding beta-tubulin binding gap junction hemi-channel activity gap junction channel activity involved in cardiac conduction electrical coupling scaffold protein binding gap junction channel activity involved in cell communication by electrical coupling protein tyrosine kinase binding channel activity SH3 domain binding protein domain specific binding PDZ domain binding connexin binding disordered domain specific binding
Process(es)
establishment of mitotic spindle orientation osteoblast differentiation in utero embryonic development neuron migration negative regulation of endothelial cell proliferation heart looping epithelial cell maturation lens development in camera-type eye chronic inflammatory response positive regulation of glomerular filtration atrial ventricular junction remodeling epicardial cell to mesenchymal cell transition cell communication signal transduction positive regulation of cytosolic calcium ion concentration cell-cell signaling spermatogenesis heart development adult heart development regulation of heart contraction protein localization negative regulation of cell proliferation male gonad development response to pH vascular transport positive regulation of gene expression negative regulation of gene expression cell communication by chemical coupling cell communication by electrical coupling regulation of cell communication by electrical coupling positive regulation of cell communication by chemical coupling glutamate secretion ATP transport negative regulation of cell growth regulation of bone mineralization positive regulation of insulin secretion negative regulation of gonadotropin secretion ion transmembrane transport glutathione transmembrane transport embryonic heart tube development maintenance of protein localization in endoplasmic reticulum T cell proliferation T cell activation vasodilation embryonic digit morphogenesis xenobiotic transport regulation of apoptotic process skeletal muscle tissue regeneration cell-cell junction organization positive regulation of protein catabolic process positive regulation of meiotic nuclear division positive regulation of striated muscle tissue development positive regulation of vasoconstriction bone remodeling regulation of bone remodeling blood vessel morphogenesis neuron projection morphogenesis regulation of body fluid levels regulation of calcium ion transport transmembrane transport negative regulation of cardiac muscle cell proliferation milk ejection regulation of ventricular cardiac muscle cell membrane repolarization regulation of blood vessel remodeling bone development regulation of atrial cardiac muscle cell membrane depolarization regulation of ventricular cardiac muscle cell membrane depolarization cardiac conduction cellular response to pH cell communication by electrical coupling involved in cardiac conduction establishment of epithelial cell polarity microtubule-based transport regulation of actin filament organization positive regulation of cold-induced thermogenesis export across plasma membrane negative regulation of trophoblast cell migration regulation of blood circulation positive regulation of establishment of Sertoli cell barrier cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation positive regulation of morphogenesis of an epithelium positive regulation of mesodermal cell differentiation negative regulation of DNA biosynthetic process positive regulation of stem cell proliferation regulation of bicellular tight junction assembly positive regulation of behavioral fear response response to ischemia endothelium development response to wounding response to glucose gap junction assembly response to estradiol response to lipopolysaccharide response to retinoic acid response to fluid shear stress cellular response to platelet-derived growth factor stimulus response to peptide hormone decidualization cellular response to mechanical stimulus cellular response to parathyroid hormone stimulus positive regulation of smooth muscle cell chemotaxis epididymis development cardiac conduction system development maintenance of blood-brain barrier positive regulation of I-kappaB kinase/NF-kappaB signaling atrial cardiac muscle cell action potential
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