Search
Invitrogen
NEFL Monoclonal Antibody (DA2)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 15
NEFL Antibody (13-0400) in IHC (P)
Immunohistochemistry analysis of the neurofilament light chain showing staining in the filaments of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 m... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-0400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
DA2
Immunogen
Specifically recognizes a phosphate-independent epitope on NF-L.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
purified
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
13-0400 detects the neurofilament, light chain in human, rat, and mouse samples.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 68 kDa neurofilament protein; 68kDa neurofilament; FLJ53642; intermediate filament; light molecular weight neurofilament protein; N-FL; NEF3; NEFL; neurofilament; neurofilament 3; neurofilament protein; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110; unnamed protein product
Gene Aliases: CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110
UniProt ID: (Human) P07196, (Rat) P19527, (Mouse) P08551
Entrez Gene ID: (Human) 4747, (Rat) 83613, (Mouse) 18039
structural constituent of cytoskeleton
protein binding
tubulin binding
protein domain specific binding
protein binding, bridging
identical protein binding
phospholipase binding
macromolecular complex binding
structural constituent of postsynaptic intermediate filament cytoskeleton
molecular condensate scaffold activity
anterograde axonal transport
retrograde axonal transport
response to toxic substance
axonal transport of mitochondrion
spinal cord development
hippocampus development
cerebral cortex development
neurofilament bundle assembly
response to peptide hormone
intermediate filament polymerization or depolymerization
intermediate filament organization
protein polymerization
response to corticosterone
regulation of synapse maturation
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
response to sodium arsenite
response to acrylamide
microtubule cytoskeleton organization
peripheral nervous system axon regeneration
regulation of axon diameter
locomotion
negative regulation of neuron apoptotic process
intermediate filament polymerization
intermediate filament bundle assembly
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
neurofilament cytoskeleton organization
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support